Cellular agriculture could meet growing demand for animal products, but
yields are typically low and regulatory bodies restrict genetic
modification for cultured meat production. Here we demonstrate the
spontaneous immortalization and genetic stability of fibroblasts derived
from several chicken breeds. Cell lines were adapted to grow as
single-cell suspensions using serum-free culture medium, reaching
densities of 108 × 106 cells per ml in continuous culture, corresponding
to yields of 36% w/v. …
