We present a method of producing bulk cell-cultured fat tissue for food
applications. Mass transport limitations (nutrients, oxygen, waste
diffusion) of macroscale 3D tissue culture are circumvented by initially
culturing murine or porcine adipocytes in 2D, after which bulk fat
tissue is produced by mechanically harvesting and aggregating the
lipid-filled adipocytes into 3D constructs using alginate or
transglutaminase binders. The 3D fat tissues were visually similar to
fat tissue harvested …
