We present a method of producing bulk cell-cultured fat tissue for food
applications. Mass transport limitations (nutrients, oxygen, waste
diffusion) of macroscale 3D tissue culture are circumvented by initially
culturing murine or porcine adipocytes in 2D, after which bulk fat is
produced by mechanically harvesting and aggregating the lipid-filled
adipocytes into 3D fats using alginate or transglutaminase binders. The
3D fats were visually similar to fat tissue harvested from animals, with …
